Exploring Antibody Internalization: Case Studies Using the iQue® HTS Platform

Learn how pH-sensitive probe–based assays and multiplexed cytometry analysis can be applied to quantitatively evaluate antibody internalization across multiple cell types in a high-throughput workflow.

In this application note, you’ll learn how to:

• Perform antibody internalization assays using a no-wash, pH-sensitive probe–based method
• Analyze multiple cell populations simultaneously using multiplexing with encoding dyes
• Evaluate the impact of antibody isotypes and immune cell differentiation on internalization
• Characterize antibody-drug conjugates (ADCs) for binding, internalization, and cytotoxicity

What’s Inside the Application Note:

This application note walks through a streamlined experimental workflow, including:

• ABI assay workflow using a pH-sensitive probe for accurate internalization measurement
• Multiplexed cytometry approach for analyzing different cell types within a single well
• Case study data demonstrating how isotype variation and cell differentiation influence internalization
• Comparative analysis of ADCs such as Trastuzumab, Kadcyla, and Enhertu