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Membrane Protein Expression by VLP Platform
Membrane proteins play a vital role in cellular activities, so much so that their abnormal function is related to many human diseases. In addition, membrane proteins are important drug targets, but its production is challenging due to factors such as low expression, difficult preparation, and poor stability.
Membrane proteins play a vital role in cellular activities, so much so that their abnormal function is related to many human diseases. In addition, membrane proteins are important drug targets, but its production is challenging due to factors such as low expression, difficult preparation, and poor stability. Our virus-like particle (VLP) technology platform based on the HEK293 expression system allows displaying the target membrane protein on the VLP surface, allowing its detection as a normal protein. For instance, our full-length membrane protein product Claudin 18.2, GPRC5D, and Claudin 6 have shown adequate activity levels. Sino Biological provides custom membrane protein services to facilitate the drug development process.
The process of VLP displaying target protein
Service Highlights
High Speed
As fast as 3–4 weeks to get
deliverables
Great Bioactivity
Expression in HEK293 cells,
similar to native structure
Various Applications
Immunization, ELISA,
SPR/BLI, PK/PD, etc.
Service Workflow
| Step and Content | Timeline | Deliverables |
 Gene synthesis and codon optimization (optional)• Simply provide us with the protein/gene sequence or plasmid |
2 weeks | • 50-200 μg purified proteins (if feasible) • CoA: > BCA and WB > SEC-HPLC (optional) > ELISA (optional) |
| Vector construction • Cloning into optimized and efficient expression vector • Plasmid sequencing and large-scale plasmid preparation |
1-2 weeks | |
| Pilot-scale protein expression and purification • Transient transfection of HEK293 cells • VLP protein purification • QC analysis |
2-3 weeks | |
| Large-scale expression and purification (optional) • Protein expression and purification • QC analysis |
3-4 weeks | • Purified proteins • CoA |
Case Display: Claudin 6-VLP Protein
Sino Biological has developed a wide variety of full-length transmembrane proteins using the VLP-based membrane protein expression platform. The activity of these membrane proteins has been validated.
Full-length Human Claudin 6-VLP Protein (Cat#: 16658-HNAH)
High Activity
High batch-to-batch Consistency
High Freeze-thaw Stability
