Custom Recombinant Protein Expression Services

With 1,000+ new proteins in R&D each year, Sino Biological has extensive experience in producing recombinant proteins such as transmembrane proteins, enzymes, kinases, viral antigens, etc. We provide complete services from gene cloning and synthesis to protein characterization. Each project is designed with checkpoints that can provide you with decision-informing data before proceeding to the next step.

Multiple protein expression systems are available including those of insect cells, mammalian cells, and E. coli. We are dedicated to helping

6,000+
Proteins successfully developed

80+
Bioreactors ranging from 2 to 1500 L

μg-kg Level
Flexible production scales

4 Weeks
Fast turnaround in HEK293 cells

Featured Case Studies of Protein Production Services

Case #1: Tag-free homodimer protein expressed in HEK293 cells

Project: Tag-free protein

Requirements: 10 mg, purity>95% by SEC-HPLC and SDS-PAGE

Service: Mammalian transient expression service

SEC-HPLC and SDS-PAGE Analysis of Target Protein after IEC and SEC

          

                                                                                    Lane M: Marker

                                                                                    Lane 1: Reducing
                                                                                    Lane 2: Non-reducing

 

 Case #2: Single-pass membrane proteins expressed in insect cells

Project: Single-pass membrane protein (enzyme)

Requirements: Protein extraction optimization and two-step protein purification

Service: Baculovirus-Insect Expression Service

Protein Extraction

Lane 1: Total protein from cell lysate

Lane 2: Extraction condition 1

Lane 3: Extraction condition 2 (optimized)

 

 

Protein Extraction

Lane M: Marker

Lane 1: Sample after protein extraction

Lane 2: Sample after SP column purification

Lane 3: Sample after affinity chromatography