Research Insights
The Research Insights area highlights recent advances in science and medicine as well as global laboratory research. Our readers gain tremendous benefit from research discoveries in an indirect manner.
Transcriptome-wide mapping reveals a diverse dihydrouridine landscape including mRNA
Dihydrouridine is a modified nucleotide universally present in tRNAs but the complete dihydrouridine landscape is unknown in any organism We introduce dihydrouridine sequencing Dseq for transcriptomewide mapping of D with singlenucleotide resolution and use it to uncover novel classes of dihydrouridinecontaining
Comparative studies between the murine immortalized brain endothelial cell line (bEnd.3) and induced pluripotent stem cell-derived human brain endothelial cells for paracellular transport
Brain microvascular endothelial cells forming the anatomical site of the bloodbrain barrier BBB are widely used as in vitro complements to in vivo BBB studies Among the immortalized cells used as in vitro BBB models the murinederived bEnd cells offer culturing consistency and low cost and are well characterized for functional and transport assays
Designing optimal allocations for cancer screening using queuing network models
Cancer is one of the leading causes of death but mortality can be reduced by detecting tumors earlier so that treatment is initiated at a less aggressive stage The tradeoff between costs associated with screening and its benefit makes the decision of whom to screen and when a challenge To enable comparisons across screening
Integrated bioinformatics and statistical approaches to explore molecular biomarkers for breast cancer diagnosis, prognosis and therapies
Integrated bioinformatics and statistical approaches are now playing the vital role in identifying potential molecular biomarkers more accurately in presence of huge number of alternatives for disease diagnosis prognosis and therapies by reducing time and cost compared to the wetlab based experimental procedures
Generating dynamic gene expression patterns without the need for regulatory circuits
Synthetic biology has successfully advanced our ability to design and implement complex timevarying genetic circuits to control the expression of recombinant proteins However these circuits typically require the production of regulatory genes whose only purpose is to coordinate expression of other genes When designing
